Fig. 4

Scpp genes in the ocean sunfish. Upper panel: Comparison of the sunfish sparcl1 locus with those of zebrafish and fugu showing the missing Scpp genes in sunfish (fa93e10, scpp7 and scpp4). The scpp4 pseudogene in the ocean sunfish is shown as a dotted arrow. Lower panel: Alignment of sunfish, fugu and medaka scpp4 sequences showing the single base insertion in exon 2 of this gene in sunfish resulting in a premature termination codon followed by a frameshift in the rest of the open reading frame. This insertion was confirmed by PCR and sequencing of genomic DNA from two other specimens (GenBank accession numbers KF737069 and KF737070). The termination codon in sunfish is underlined in red. The accession numbers for fugu and medaka scpp4 genes used in the alignment are DQ066525.1 and XM_004065875.2, respectively. chr, chromosome; scaf, scaffold